• Western blot analysis of extracts of various cell lines, using IFI44 antibody (STJ110487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using IFI44 antibody (STJ110487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using IFI44 antibody (STJ110487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using IFI44 rabbit polyclonal antibody (STJ110487) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using IFI44 rabbit polyclonal antibody (STJ110487) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IFI44 antibody (165-181) (STJ110487)

SKU:
STJ110487

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IFI44 (165-181) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFI44
Gene ID: 10561
Uniprot ID: IFI44_HUMAN
Immunogen Region: 165-181
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 165-181 of mouse IFI44 (NP_598632.2).
Immunogen Sequence: LLQALRDYKPYGDLVQQ
Function This protein aggregates to form microtubular structures.
Protein Name Interferon-Induced Protein 44
P44
Microtubule-Associated Protein 44
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Interferon-Induced Protein 44 antibody
Anti-P44 antibody
Anti-Microtubule-Associated Protein 44 antibody
Anti-IFI44 antibody
Anti-MTAP44 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance