Anti-IER3 antibody (50-150) (STJ111301)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal IER3 (50-150) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IER3 |
| Gene ID | 8870 |
| Uniprot ID | IEX1_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | GRPSASRGHRKRSRRVLYPR VVRRQLPVEEPNPAKRLLFL LLTIVFCQILMAEEGVPAPL PPEDAPNAASLAPTPVSAVL EPFNLTSEPSDYALDLSTFL Q |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IER3 (NP_003888.2). |
Additional Info
| Post Translational Modifications | Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli. Glycosylated. |
| Function | May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Also acts as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. |
| Protein Name | Radiation-Inducible Immediate-Early Gene Iex-1Differentiation-Dependent Gene 2 ProteinProtein Dif-2Immediate Early Protein Gly96Immediate Early Response 3 ProteinPacap-Responsive Gene 1 ProteinProtein Prg1 |
| Database Links | Reactome: R-HSA-6811558 |
| Cellular Localisation | MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Radiation-Inducible Immediate-Early Gene Iex-1 antibodyAnti-Differentiation-Dependent Gene 2 Protein antibodyAnti-Protein Dif-2 antibodyAnti-Immediate Early Protein Gly96 antibodyAnti-Immediate Early Response 3 Protein antibodyAnti-Pacap-Responsive Gene 1 Protein antibodyAnti-Protein Prg1 antibodyAnti-IER3 antibodyAnti-DIF2 antibodyAnti-IEX1 antibodyAnti-PRG1 antibody |
Information sourced from Uniprot.org