• Western blot analysis of the lysates from HeLa cells using ID4 antibody.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from HepG2 cells, using ID4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Id4 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ID4 antibody (1-50 aa) (STJ93637)

SKU:
STJ93637

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA-binding protein inhibitor ID-4 (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ID4
Gene ID: 3400
Uniprot ID: ID4_HUMAN
Immunogen Region: 1-50 aa
Specificity: Id4 Polyclonal Antibody detects endogenous levels of Id4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ID4 at the amino acid range 1-50
Protein Name Dna-Binding Protein Inhibitor Id-4
Class B Basic Helix-Loop-Helix Protein 27
Bhlhb27
Inhibitor Of Dna Binding 4
Inhibitor Of Differentiation 4
Database Links Reactome: R-HSA-9031628
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna-Binding Protein Inhibitor Id-4 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 27 antibody
Anti-Bhlhb27 antibody
Anti-Inhibitor Of Dna Binding 4 antibody
Anti-Inhibitor Of Differentiation 4 antibody
Anti-ID4 antibody
Anti-BHLHB27 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance