| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ICOS |
| Gene ID: |
29851 |
| Uniprot ID: |
ICOS_HUMAN |
| Immunogen Region: |
21-140 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 21-140 of human ICOS/CD278 (NP_036224.1). |
| Immunogen Sequence: |
EINGSANYEMFIFHNGGVQI LCKYPDIVQQFKMQLLKGGQ ILCDLTKTKGSGNTVSIKSL KFCHSQLSNNSVSFFLYNLD HSHANYYFCNLSIFDPPPFK VTLTGGYLHIYESQLCCQLK |
| Tissue Specificity | Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus. |
| Post Translational Modifications | N-glycosylated. |
| Function | Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Acts also as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. |
| Protein Name | Inducible T-Cell CostimulatorActivation-Inducible Lymphocyte Immunomediatory MoleculeCd Antigen Cd278 |
| Database Links | Reactome: R-HSA-1257604 Q9Y6W8-1Reactome: R-HSA-2219530 Q9Y6W8-1Reactome: R-HSA-388841 Q9Y6W8-1Reactome: R-HSA-6811558 Q9Y6W8-1Reactome: R-HSA-9725371 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative Antibody Names | Anti-Inducible T-Cell Costimulator antibodyAnti-Activation-Inducible Lymphocyte Immunomediatory Molecule antibodyAnti-Cd Antigen Cd278 antibodyAnti-ICOS antibodyAnti-AILIM antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
