• Western blot analysis of 293F-IAPP, using IAPP rabbit polyclonal antibody (STJ24112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded Rat pancreas using IAPP rabbit polyclonal antibody (STJ24112) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreas using IAPP rabbit polyclonal antibody (STJ24112) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human pancreas cells using IAPP rabbit polyclonal antibody (STJ24112) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse pancreas cells using IAPP rabbit polyclonal antibody (STJ24112) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IAPP antibody (1-89) (STJ24112)

SKU:
STJ24112

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IAPP (1-89) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IAPP
Gene ID: 3375
Uniprot ID: IAPP_HUMAN
Immunogen Region: 1-89
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-89 of human IAPP (NP_000406.1).
Immunogen Sequence: MGILKLQVFLIVLSVALNHL KATPIESHQVEKRKCNTATC ATQRLANFLVHSSNNFGAIL SSTNVGSNTYGKRNAVEVLK REPLNYLPL
Post Translational Modifications Amyloid fibrils are degraded by IDE.
Function Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
Protein Name Islet Amyloid Polypeptide
Amylin
Diabetes-Associated Peptide
Dap
Insulinoma Amyloid Peptide
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-418555
Reactome: R-HSA-419812
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Islet Amyloid Polypeptide antibody
Anti-Amylin antibody
Anti-Diabetes-Associated Peptide antibody
Anti-Dap antibody
Anti-Insulinoma Amyloid Peptide antibody
Anti-IAPP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance