• Confocal imaging of HeLa cells using HSP60/HSPD1 Rabbit monoclonal antibody (STJ11101241, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HSP60/HSPD1 Rabbit monoclonal antibody (STJ11101241) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using HSP60/HSPD1 Rabbit monoclonal antibody (STJ11101241) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HSPD1 antibody (350-450) [S1MR] (STJ11101241)

SKU:
STJ11101241

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hsp60 (350-450) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSPD1
Gene ID: 3329
Uniprot ID: CH60_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human HSP60/HSPD1 (P10809).
Immunogen Sequence: VTKDDAMLLKGKGDKAQIEK RIQEIIEQLDVTTSEYEKEK LNERLAKLSDGVAVLKVGGT SDVEVNEKKDRVTDALNATR AAVEEGIVLGGGCALLRCIP A
Function Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Protein Name 60 Kda Heat Shock Protein - Mitochondrial
60 Kda Chaperonin
Chaperonin 60
Cpn60
Heat Shock Protein 60
Hsp-60
Hsp60
Hucha60
Mitochondrial Matrix Protein P1
P60 Lymphocyte Protein
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-8869496
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-60 Kda Heat Shock Protein - Mitochondrial antibody
Anti-60 Kda Chaperonin antibody
Anti-Chaperonin 60 antibody
Anti-Cpn60 antibody
Anti-Heat Shock Protein 60 antibody
Anti-Hsp-60 antibody
Anti-Hsp60 antibody
Anti-Hucha60 antibody
Anti-Mitochondrial Matrix Protein P1 antibody
Anti-P60 Lymphocyte Protein antibody
Anti-HSPD1 antibody
Anti-HSP60 antibody

Information sourced from Uniprot.org

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