• Immunofluorescence analysis of L929 cells using HSPB11 Rabbit polyclonal antibody (STJ11100181) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HSPB11 in paraffin-embedded mouse lung using HSPB11 Rabbit polyclonal antibody (STJ11100181) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of HSPB11 in paraffin-embedded human placenta using HSPB11 Rabbit polyclonal antibody (STJ11100181) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HSPB11 Rabbit polyclonal antibody (STJ11100181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-HSPB11 antibody (1-144) (STJ11100181)

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STJ11100181

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSPB11 (1-144) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFT25
Gene ID: 51668
Uniprot ID: IFT25_HUMAN
Immunogen Region: 1-144
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human HSPB11 (NP_057210.2).
Immunogen Sequence: MRKIDLCLSSEGSEVILATS SDEKHPPENIIDGNPETFWT TTGMFPQEFIICFHKHVRIE RLVIQSYFVQTLKIEKSTSK EPVDFEQWIEKDLVHTEGQL QNEEIVAHDGSATYLRFIIV SAFDHFASVHSVSAEGTVVS NLSS
Tissue Specificity Detected in placenta.
Function Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumulation of GLI2 at the ciliary tip in response to activation of the SHH pathway, suggesting it is involved in the dynamic transport of SHH signaling molecules within the cilium. Not required for ciliary assembly. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation. Plays a role in the early development of the kidney. May be involved in the regulation of ureteric bud initiation.
Protein Name Intraflagellar Transport Protein 25 Homolog
Heat Shock Protein Beta-11
Hspb11
Placental Protein 25
Pp25
Database Links Reactome: R-HSA-5620924
Cellular Localisation Cell Projection
Cilium
Alternative Antibody Names Anti-Intraflagellar Transport Protein 25 Homolog antibody
Anti-Heat Shock Protein Beta-11 antibody
Anti-Hspb11 antibody
Anti-Placental Protein 25 antibody
Anti-Pp25 antibody
Anti-IFT25 antibody
Anti-C1orf41 antibody
Anti-HSPB11 antibody
Anti-HSPC034 antibody

Information sourced from Uniprot.org

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