| Host: | Mouse |
| Applications: | ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-HSP40/DNAJB1 (1-340aa) for use in ELISA and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | k1C7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2ak |
| Formulation: | Liquid in phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
| Purification: | By protein-G affinity chromatography |
| Concentration: | 1 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DNAJB1 |
| Gene ID: | 3337 |
| Uniprot ID: | DNJB1_HUMAN |
| Immunogen Region: | 1-340aa |
| Accession Number: | NM_006145 |
| Immunogen: | Recombinant human Hsp40 (1-340aa) purified from E. coli |
| Function | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro). |
| Protein Name | Dnaj Homolog Subfamily B Member 1Dnaj Protein Homolog 1Heat Shock 40 Kda Protein 1Hsp40Heat Shock Protein 40Human Dnaj Protein 1Hdj-1 |
| Database Links | Reactome: R-HSA-3371453Reactome: R-HSA-3371497Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-5687128 |
| Cellular Localisation | CytoplasmNucleusNucleolusTranslocates Rapidly From The Cytoplasm To The NucleusAnd Especially To The NucleoliUpon Heat Shock |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily B Member 1 antibodyAnti-Dnaj Protein Homolog 1 antibodyAnti-Heat Shock 40 Kda Protein 1 antibodyAnti-Hsp40 antibodyAnti-Heat Shock Protein 40 antibodyAnti-Human Dnaj Protein 1 antibodyAnti-Hdj-1 antibodyAnti-DNAJB1 antibodyAnti-DNAJ1 antibodyAnti-HDJ1 antibodyAnti-HSPF1 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/101151/388526/STJ27455_1__36817.1713158040.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] DNAJB1 Rabbit polyclonal antibody (STJ27455) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")