• Western blot analysis of extracts of various cell lines, using HSD17B13 antibody (STJ28012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using HSD17B13 rabbit polyclonal antibody (STJ28012) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using HSD17B13 rabbit polyclonal antibody (STJ28012) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human liver cells using HSD17B13 rabbit polyclonal antibody (STJ28012) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse liver cells using HSD17B13 rabbit polyclonal antibody (STJ28012) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver cells using HSD17B13 rabbit polyclonal antibody (STJ28012) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-HSD17B13 antibody (30-211) (STJ28012)

SKU:
STJ28012

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HSD17B13 (30-211) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HSD17B13
Gene ID: 345275
Uniprot ID: DHB13_HUMAN
Immunogen Region: 30-211
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-211 of human HSD17B13 (NP_835236.2).
Immunogen Sequence: RRKSVAGEIVLITGAGHGIG RQTTYEFAKRQSILVLWDIN KRGVEETAAECRKLGVTAHA YVVDCSNREEIYRSLNQVKK EVGDVTIVVNNAGTVYPADL LSTKDEEITKTFEVNILGHF WITKALLPSMMERNHGHIVT VASVCGHEGIPYLIPYCSSK FAAVGFHRGLTSELQALGKT GI
Tissue Specificity Highly expressed in the liver. Also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis.
Function Plays a pivotal role in hepatic lipid metabolism. In vitro, it catalyzes the oxidation of a variety of lipid substrates, including 17beta-estradiol, retinol, retinal, and leukotriene B4. Isoform 2: Has retinol/retinal dehydrogenase activity in vitro. Isoform 1: Does not have retinol/retinal dehydrogenase activity in vitro.
Protein Name 17-Beta-Hydroxysteroid Dehydrogenase 13
17-Beta-Hsd 13
Hepatic Retinol/Retinal Dehydrogenase
Short Chain Dehydrogenase/Reductase Family 16c Member 3
Short-Chain Dehydrogenase/Reductase 9
Database Links Reactome: R-HSA-8964572
Cellular Localisation Isoform 2: Lipid Droplet
Endoplasmic Reticulum
Redistributed From The Endoplasmic Reticulum To Lipids Droplets In The Cell Upon Induction Of Lipids Droplet Formation
Isoform 1: Cytoplasm
Does Not Localize To Lipid Droplets
Alternative Antibody Names Anti-17-Beta-Hydroxysteroid Dehydrogenase 13 antibody
Anti-17-Beta-Hsd 13 antibody
Anti-Hepatic Retinol/Retinal Dehydrogenase antibody
Anti-Short Chain Dehydrogenase/Reductase Family 16c Member 3 antibody
Anti-Short-Chain Dehydrogenase/Reductase 9 antibody
Anti-HSD17B13 antibody
Anti-SCDR9 antibody
Anti-SDR16C3 antibody
Anti-HMFN0376 antibody
Anti-UNQ497 antibody
Anti-PRO1014 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance