Confocal imaging of NIH/3T3 cells usingย 15-PGDH/HPGD Rabbit monoclonal antibody (STJ11102225, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-HPGD antibody (50-151) [S5MR] (STJ11102225)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102225
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General Information

Short DescriptionRabbit monoclonal PGDH (50-151) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolHPGD
Gene ID3248
Uniprot IDPGDH_HUMAN
Immunogen Region50-151
Immunogen SequenceDEQFEPQKTLFIQCDVADQQ QLRDTFRKVVDHFGRLDILV NNAGVNNEKNWEKTLQINLV SVISGTYLGLDYMSKQNGGE GGIIINMSSLAGLMPVAQQP VY
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 50-151 of human 15-PGDH/HPGD (P15428).

Additional Info

Tissue Specificity Detected in colon epithelium (at protein level).
Function Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites. Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.
Protein Name 15-Hydroxyprostaglandin Dehydrogenase Nad(+
15-Pgdh
Eicosanoid/Docosanoid Dehydrogenase Nad(+
Prostaglandin Dehydrogenase 1
Short Chain Dehydrogenase/Reductase Family 36c Member 1
Database Links Reactome: R-HSA-2142700
Reactome: R-HSA-2162123
Reactome: R-HSA-9018676
Reactome: R-HSA-9018896
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-15-Hydroxyprostaglandin Dehydrogenase Nad(+ antibody
Anti-15-Pgdh antibody
Anti-Eicosanoid/Docosanoid Dehydrogenase Nad(+ antibody
Anti-Prostaglandin Dehydrogenase 1 antibody
Anti-Short Chain Dehydrogenase/Reductase Family 36c Member 1 antibody
Anti-HPGD antibody
Anti-PGDH1 antibody
Anti-SDR36C1 antibody

Information sourced from Uniprot.org

Citations

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