• Western blot analysis of extracts of various cell lines, using Haptoglobin (HP) (HP) rabbit monoclonal antibody (STJ11102373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
  • Immunofluorescence analysis of rat liver using Haptoglobin (HP) (HP) Rabbit monoclonal antibody (STJ11102373) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using Haptoglobin (HP) (HP) rabbit monoclonal antibody (STJ11102373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using Haptoglobin (HP) (HP) Rabbit monoclonal antibody (STJ11102373) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat liver using Haptoglobin (HP) (HP) rabbit monoclonal antibody (STJ11102373) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Haptoglobin (HP) (HP) Rabbit monoclonal antibody (STJ11102373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-HP antibody (307-406) [S3MR] (STJ11102373)

SKU:
STJ11102373

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Haptoglobin (307-406) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HP
Gene ID: 3240
Uniprot ID: HPT_HUMAN
Immunogen Region: 307-406
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 307-406 of human Haptoglobin (HP) (P00738).
Immunogen Sequence: DQCIRHYEGSTVPEKKTPKS PVGVQPILNEHTFCAGMSKY QEDTCYGDAGSAFAVHDLEE DTWYATGILSFDKSCAVAEY GVYVKVTSIQDWVQKTIAEN
Tissue Specificity Expressed by the liver and secreted in plasma.
Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Protein Name Haptoglobin
Zonulin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain
Database Links Reactome: R-HSA-2168880
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative Antibody Names Anti-Haptoglobin antibody
Anti-Zonulin Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain antibody
Anti-HP antibody

Information sourced from Uniprot.org

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