STJ71423 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Hornerin antibody (Internal) (STJ71423)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71423-100
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General Information

Short DescriptionGoat polyclonal antibody anti-Hornerin (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/FC
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
IF: Strong expression of the protein seen in the cytoplasm and plasma membrane of U251 cells. Recommended concentration: 10ยตg/ml.

FC:Flow cytometric analysis of A549 cells. Recommended c

Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolHRNR
Gene ID388697
Uniprot IDHORN_HUMAN
Accession NumberNP_001009931.1
Immunogen RegionInternal
Immunogen SequenceQYATQHGEYDTLNK

Additional Info

Post Translational Modifications Processed during the process of epidermal differentiation. Forms covalent cross-links mediated by transglutaminase TGM3, between glutamine and the epsilon-amino group of lysine residues (in vitro).
Function Component of the epidermal cornified cell envelopes.
Protein Name Hornerin
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasmic Granule
Found In Keratohyalin Granules In The Granular Cells Of The Epidermis
Alternative Antibody Names Anti-Hornerin antibody
Anti-HRNR antibody
Anti-S100A18 antibody

Information sourced from Uniprot.org

Citations

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