• Western blot analysis of extracts of various cell lines, using HNRNPR antibody (STJ29924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat heart using HNRNPR antibody (STJ29924) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human esophagus using HNRNPR antibody (STJ29924) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse liver using HNRNPR antibody (STJ29924) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of U2OS cells using HNRNPR antibody (STJ29924) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HNRNPR antibody (C-Term) (STJ29924)

SKU:
STJ29924

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HNRNPR (C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HNRNPR
Gene ID: 10236
Uniprot ID: HNRPR_HUMAN
Immunogen Region: C-Term
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human HNRNPR (NP_005817.1).
Immunogen Sequence: RGRGGGRGGRGAPPPPRGRG APPPRGRAGYSQRGAPLGPP RGSRGGRGGPAQQQRGRGSR GSRGNRGGNVGGKRKADGYN QPDSKRRQTNNQQNWGSQPI AQQPLQQGGDYSGNYGYNND NQEFYQDTYGQQWK
Function Component of ribonucleosomes, which are complexes of at least 20 other different heterogeneous nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.
Protein Name Heterogeneous Nuclear Ribonucleoprotein R
Hnrnp R
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72203
Cellular Localisation Nucleus
Microsome
Nucleoplasm
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
The Tyrosine Phosphorylated Form Bound To Rna Is Found In Microsomes
Alternative Antibody Names Anti-Heterogeneous Nuclear Ribonucleoprotein R antibody
Anti-Hnrnp R antibody
Anti-HNRNPR antibody
Anti-HNRPR antibody

Information sourced from Uniprot.org

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