Anti-hnRNP C1/C2 antibody [R08-6F5] (STJA0036034)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human hnRNP C1/C2
General Information
| Short Description | Rabbit monoclonal anti-hnRNP C1/C2 for use in WB, IHC-P, ICC, IF, IP and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-6F5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | HNRNPC |
| Gene ID | 3183 |
| Uniprot ID | HNRPC_HUMAN |
| Immunogen | A synthesized peptide derived from human hnRNP C1/C2 |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide. Sumoylated. Sumoylation reduces affinity for mRNA. Ubiquitinated and degraded after nucleo-cytoplasmic transport by YWHAE. |
| Function | Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing. |
| Protein Name | Heterogeneous Nuclear Ribonucleoproteins C1/C2Hnrnp C1/C2 |
| Database Links | Reactome: R-HSA-4570464Reactome: R-HSA-72163Reactome: R-HSA-72203Reactome: R-HSA-9013418Reactome: R-HSA-9013422 |
| Cellular Localisation | NucleusComponent Of Ribonucleosomes |
| Alternative Antibody Names | Anti-Heterogeneous Nuclear Ribonucleoproteins C1/C2 antibodyAnti-Hnrnp C1/C2 antibodyAnti-HNRNPC antibodyAnti-HNRPC antibody |
Information sourced from Uniprot.org