• Western blot analysis of lysate from A549 cells, using FOXA1 Antibody.
  • Western blot analysis of A549 cells using HNF-3 Alpha/Beta/Gamma Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HNF-Alpha/beta/gamma antibody (STJ96689)

SKU:
STJ96689

Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-HNF-Alpha/beta/gamma antibody is suitable for use in Western Blot and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Specificity: HNF-3 alpha/beta/gamma polyclonal antibody detects endogenous levels of HNF-3 alpha/beta/gamma protein.
Uniprot ID: FOXA1_HUMAN
Gene ID: 3169
Gene Symbol: FOXA1
Immunogen: Synthesized peptide derived from human HNF-3alpha/beta/gamma around the non-acetylation site of K265.
Function Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-ACAATTAGTTGTAGCTTCT-3'. Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate.FOXA1 and FOXA2 seem to have at least in part redundant roles. Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription.required for ESR1 binding to the NKX2-1 promoter in breast cancer cells.binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
Protein Name Hepatocyte Nuclear Factor 3-Alpha
Hnf-3-Alpha
Hnf-3a
Forkhead Box Protein A1
Transcription Factor 3a
Tcf-3a
Database Links Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 3-Alpha antibody
Anti-Hnf-3-Alpha antibody
Anti-Hnf-3a antibody
Anti-Forkhead Box Protein A1 antibody
Anti-Transcription Factor 3a antibody
Anti-Tcf-3a antibody
Anti-FOXA1 antibody
Anti-HNF3A antibody
Anti-TCF3A antibody

Information sourced from Uniprot.org

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