• Western blot analysis of lysates from HT-29 cells, using HNF4 alpha Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using HNF-4 Alpha Polyclonal Antibody diluted at 1:2000

Anti-HNF-Alpha antibody (STJ93554)

SKU:
STJ93554

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-HNF-Alpha antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity: HNF-4 alpha polyclonal antibody detects endogenous levels of HNF-4 alpha protein.
Uniprot ID: HNF4A_HUMAN
Gene ID: 3172
Gene Symbol: HNF4A
Immunogen: Synthesized peptide derived from human HNF4-alpha around the non-phosphorylation site of S313.
Immunogen Region: 250-330 aa
Function Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Protein Name Hepatocyte Nuclear Factor 4-Alpha
Hnf-4-Alpha
Nuclear Receptor Subfamily 2 Group A Member 1
Transcription Factor 14
Tcf-14
Transcription Factor Hnf-4
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-383280
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 4-Alpha antibody
Anti-Hnf-4-Alpha antibody
Anti-Nuclear Receptor Subfamily 2 Group A Member 1 antibody
Anti-Transcription Factor 14 antibody
Anti-Tcf-14 antibody
Anti-Transcription Factor Hnf-4 antibody
Anti-HNF4A antibody
Anti-HNF4 antibody
Anti-NR2A1 antibody
Anti-TCF14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance