• Western blot analysis of extracts of various cell lines, using HMGCL antibody (STJ110355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using HMGCL antibody (STJ110355) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using HMGCL antibody (STJ110355) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HMGCL antibody (STJ110355) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using HMGCL antibody (STJ110355) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using HMGCL antibody (STJ110355) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human kidney using HMGCL antibody (STJ110355) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using HMGCL antibody (STJ110355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-HMGCL antibody (1-325) (STJ110355)

SKU:
STJ110355

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMGCL (1-325) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMGCL
Gene ID: 3155
Uniprot ID: HMGCL_HUMAN
Immunogen Region: 1-325
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human HMGCL (NP_000182.2).
Immunogen Sequence: MAAMRKALPRRLVGLASLRA VSTSSMGTLPKRVKIVEVGP RDGLQNEKNIVSTPVKIKLI DMLSEAGLSVIETTSFVSPK WVPQMGDHTEVLKGIQKFPG INYPVLTPNLKGFEAAVAAG AKEVVIFGAASELFTKKNIN CSIEESFQRFDAILKAAQSA NISVRGYVSCALGCPYEGKI SPAKVAEVTKKFYSMGCYEI SLGDTIGVGTPGIMKDMLSA VMQEVPLAALAVHCHDTYG
Tissue Specificity Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%).
Function Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.
Protein Name Hydroxymethylglutaryl-Coa Lyase - Mitochondrial
Hl
Hmg-Coa Lyase
3-Hydroxy-3-Methylglutarate-Coa Lyase
Database Links Reactome: R-HSA-77111
Reactome: R-HSA-9033241
Cellular Localisation Mitochondrion Matrix
Peroxisome
Unprocessed Form Is Peroxisomal
Alternative Antibody Names Anti-Hydroxymethylglutaryl-Coa Lyase - Mitochondrial antibody
Anti-Hl antibody
Anti-Hmg-Coa Lyase antibody
Anti-3-Hydroxy-3-Methylglutarate-Coa Lyase antibody
Anti-HMGCL antibody

Information sourced from Uniprot.org

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