Anti-HMGB3 antibody (1-100) (STJ117258)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HMGB3 (1-100) antibody for use in WB and ELISA in rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HMGB3 |
| Gene ID | 3149 |
| Uniprot ID | HMGB3_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MAKGDPKKPKGKMSAYAFFV QTCREEHKKKNPEVPVNFAE FSKKCSERWKTMSGKEKSKF DEMAKADKVRYDREMKDYGP AKGGKKKKDPNAPKRPPSGF |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGB3 (NP_005333.2). |
Additional Info
| Tissue Specificity | Expressed predominantly in placenta. |
| Post Translational Modifications | Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-104 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB3 (HMGB3C23hC45hC104h), 2- disulfide HMGB3 (HMGB3C23-C45C104h) and 3- sulfonyl HMGB3 (HMGB3C23soC45soC104so). |
| Function | Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA structures such as single-stranded DNA. Can bend DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling. |
| Protein Name | High Mobility Group Protein B3High Mobility Group Protein 2aHmg-2aHigh Mobility Group Protein 4Hmg-4 |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasm |
| Alternative Antibody Names | Anti-High Mobility Group Protein B3 antibodyAnti-High Mobility Group Protein 2a antibodyAnti-Hmg-2a antibodyAnti-High Mobility Group Protein 4 antibodyAnti-Hmg-4 antibodyAnti-HMGB3 antibodyAnti-HMG2A antibodyAnti-HMG4 antibody |
Information sourced from Uniprot.org