• Immunofluorescence analysis of A-549 cells using HMG20A antibody (STJ29425).
  • Western blot analysis of various lysates, using HMG20A antibody (STJ29425) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMG20A antibody (1-347) (STJ29425)

SKU:
STJ29425

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HMG20A (1-347) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HMG20A
Gene ID: 10363
Uniprot ID: HM20A_HUMAN
Immunogen Region: 1-347
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-347 of human HMG20A (NP_060670.1).
Immunogen Sequence: MENLMTSSTLPPLFADEDGS KESNDLATTGLNHPEVPYSS GATSSTNNPEFVEDLSQGQL LQSESSNAAEGNEQRHEDEQ RSKRGGWSKGRKRKKPLRDS NAPKSPLTGYVRFMNERREQ LRAKRPEVPFPEITRMLGNE WSKLPPEEKQRYLDEADRDK ERYMKELEQYQKTEAYKVFS RKTQDRQKGKSHRQDAARQA THDHEKETEVKERSVFDIPI FTEEFLNHSKAREAELRQL
Tissue Specificity Ubiquitous.
Function Plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase KMT2A/MLL1 and consequent increased methylation of histone H3 lysine 4.
Protein Name High Mobility Group Protein 20a
Hmg Box-Containing Protein 20a
Hmg Domain-Containing Protein 1
Hmg Domain-Containing Protein Hmgx1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-High Mobility Group Protein 20a antibody
Anti-Hmg Box-Containing Protein 20a antibody
Anti-Hmg Domain-Containing Protein 1 antibody
Anti-Hmg Domain-Containing Protein Hmgx1 antibody
Anti-HMG20A antibody
Anti-HMGX1 antibody
Anti-HMGXB1 antibody

Information sourced from Uniprot.org

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