• Western blot analysis of extracts of various cell lines, using HK3 antibody (STJ110726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of A-549 cells using HK3 rabbit polyclonal antibody (STJ110726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using HK3 rabbit polyclonal antibody (STJ110726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using HK3 rabbit polyclonal antibody (STJ110726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using HK3 rabbit polyclonal antibody (STJ110726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HK3 antibody (1-270) (STJ110726)

SKU:
STJ110726

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HK3
Gene ID: 3101
Uniprot ID: HXK3_HUMAN
Immunogen Region: 1-270
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HK3 (NP_002106.2).
Immunogen Sequence: MDSIGSSGLRQGEETLSCSE EGLPGPSDSSELVQECLQQF KVTRAQLQQIQASLLGSMEQ ALRGQASPAPAVRMLPTYVG STPHGTEQGDFVVLELGATG ASLRVLWVTLTGIEGHRVEP RSQEFVIPQEVMLGAGQQLF DFAAHCLSEFLDAQPVNKQG LQLGFSFSFPCHQTGLDRST LISWTKGFRCSGVEGQDVVQ LLRDAIRRQGAYNIDVVAVV NDTVGTMMGCEPGVRPCEV
Function Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate.
Protein Name Hexokinase-3
Hexokinase Type Iii
Hk Iii
Hexokinase-C
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Alternative Antibody Names Anti-Hexokinase-3 antibody
Anti-Hexokinase Type Iii antibody
Anti-Hk Iii antibody
Anti-Hexokinase-C antibody
Anti-HK3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance