• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys12) Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of Mouse-kidney HELA lysis using Acetyl-Histone H4 (K12) antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
  • Western blot analysis of 293 cells using Acetyl-Histone H4 (K12) Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
  • Western blot analysis of KB cells using Acetyl-Histone H4 (K12) Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
  • Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys12) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Histone H4 (Acetyl K12) antibody (STJ90128)

SKU:
STJ90128
Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human,Mouse,Rat,Monkey
Short Description: Rabbit polyclonal primary antibody against Histone H4 (Acetyl K12).
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Specificity: Acetyl-Histone H4 (K12) polyclonal antibody detects endogenous levels of Histone H4 protein only when acetylated at K12.
Immunogen: Synthesized peptide derived from human Histone H4 around the acetylation site of K12
Immunogen Region: 10-90 aa
6 months standard