Western blot analysis of extracts of various cell lines, using Histone H1t antibody (STJ11100542) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Histone H1 antibody (110-209) (STJ11100542)

SKU:
STJ11100542
£112.50 - £524.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-6
Gene ID: 107970
Uniprot ID: H1T_MOUSE
Immunogen Region: 110-209
Specificity: A synthetic peptide corresponding to a sequence within amino acids 110-209 of mouse Histone H1t (NP_034507.2).
Immunogen Sequence: LSKKAASGNDKGKGKKSASA KAKKMGLPRASRSPKSSKTK AVKKPKATPTKASGSGRKTK GAKGVQQRKSPAKARAANPN SGKAKMVMQKTDLRKAAGRK
Tissue Specificity Testis-specific.
Post Translational Modifications Phosphorylated in early spermatids. Citrullination at Arg-56 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Function Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration.
Protein Name Histone H1t
Testicular H1 Histone
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H1t antibody
Anti-Testicular H1 Histone antibody
Anti-H1-6 antibody
Anti-H1f6 antibody
Anti-H1ft antibody
Anti-H1t antibody
Anti-Hist1h1t antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ118912 Anti-MXD4 antibody (110-209) (STJ118912) Rabbit Polyclonal Mouse WB/ELISA
STJ11100449 Anti-Histone H1 antibody (150-207) (STJ11100449) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/ELISA
STJ23903 Anti-Histone H1 antibody (1-105) (STJ23903) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/ELISA
STJ197462 Anti-Phospho-Histone H1-Thr3 antibody (STJ197462) Rabbit Polyclonal Human/Mouse/Rat WB
STJ197463 Anti-Phospho-Histone H1-Ser1 antibody (STJ197463) Rabbit Polyclonal Human/Mouse/Rat WB
STJ16100269 Anti-Histone H1 antibody [AE-4] (STJ16100269) Mouse Monoclonal Human/Mouse/Rat FC/IF/IHC
STJ28998 Anti-Histone H1 antibody (1-194) (STJ28998) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/IF/ICC/ELISA
STJ97160 Anti-Mono-Methyl-Histone H1-Lys25 antibody (STJ97160) Mouse Monoclonal Human/Mouse/Rat WB
STJ197302 Anti-Di-Methyl-Histone H1-Lys25 antibody (STJ197302) Rabbit Polyclonal Human/Mouse/Rat WB
!