• Confocal imaging of U-2 OS cells using Histone H1. 2 Rabbit monoclonal antibody (STJ11102702, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Western blot analysis of various lysates using Histone H1. 2 Rabbit monoclonal antibody (STJ11102702) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using Histone H1. 2 Rabbit monoclonal antibody (STJ11102702) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Histone H1.2 antibody (1-100) [S2MR] (STJ11102702)

SKU:
STJ11102702

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Histone H1.2 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-2
Gene ID: 3006
Uniprot ID: H12_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H1.2 (P16403).
Immunogen Sequence: MSETAPAAPAAAPPAEKAPV KKKAAKKAGGTPRKASGPPV SELITKAVAASKERSGVSLA ALKKALAAAGYDVEKNNSRI KLGLKSLVSKGTLVQTKGTG
Post Translational Modifications H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. ADP-ribosylated on Ser-188 in response to DNA damage.
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
Protein Name Histone H1.2
Histone H1c
Histone H1d
Histone H1s-1
Database Links Reactome: R-HSA-140342
Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Chromosome
Mainly Localizes In Euchromatin
Distribution Goes In Parallel With Dna Concentration
Alternative Antibody Names Anti-Histone H1.2 antibody
Anti-Histone H1c antibody
Anti-Histone H1d antibody
Anti-Histone H1s-1 antibody
Anti-H1-2 antibody
Anti-H1F2 antibody
Anti-HIST1H1C antibody

Information sourced from Uniprot.org

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