• Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded human esophagus tissue using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded rat colon tissue using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded rat brain tissue using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded mouse colon tissue using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded human small intestine tissue using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using Hydroxyl-Histone H2A-Y39 Rabbit monoclonal antibody (STJ11102162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-HIST1H2AE-Y39 antibody (Around Y39) [S2MR] (STJ11102162)

SKU:
STJ11102162

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hydroxyl-Histone H2A-Y39 (Around Y39) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: Around Y39
Immunogen: A synthetic hydroxylated peptide around Y39 of human Histone H2A (P04908).
Immunogen Sequence: KGNYS
Background Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis.

Information sourced from Uniprot.org

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