• Western blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000
  • Western blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-His-Tag antibody {KLH} (STJ90106)


Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Tag
Short Description: Rabbit polyclonal antibody Anti-His-Tag is suitable for use in Western Blot and ELISA.
Clonality: Polyclonal
Conjugation: KLH
Isotype: IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Concentration: 1 mg/ml
Dilution Range: WB 1:1000-1:3000
ELISA 1:20000
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Specificity: His-tag polyclonal antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
Immunogen: Synthesized peptide derived from 6X His synthetic peptide conjugated to KLH.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance