• Western blot analysis of extracts of various cell lines, using Hippocalcin (HPCA) (HPCA) Rabbit monoclonal antibody (STJ11103084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Hippocalcin antibody (1-100) [S4MR] (STJ11103084)

SKU:
STJ11103084

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hippocalcin (HPCA) (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HPCA
Gene ID: 3208
Uniprot ID: HPCA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hippocalcin (HPCA) (P84074).
Immunogen Sequence: MGKQNSKLRPEMLQDLRENT EFSELELQEWYKGFLKDCPT GILNVDEFKKIYANFFPYGD ASKFAEHVFRTFDTNSDGTI DFREFIIALSVTSRGRLEQK
Tissue Specificity Brain specific.
Post Translational Modifications Myristoylation facilitates association with membranes.
Function Calcium-binding protein that may play a role in the regulation of voltage-dependent calcium channels. May also play a role in cyclic-nucleotide-mediated signaling through the regulation of adenylate and guanylate cyclases.
Protein Name Neuron-Specific Calcium-Binding Protein Hippocalcin
Calcium-Binding Protein Bdr-2
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Association With Membranes Is Calcium-Dependent
Enriched In The Perinuclear Region
Probably At The Trans Golgi Network In Response To Calcium
Alternative Antibody Names Anti-Neuron-Specific Calcium-Binding Protein Hippocalcin antibody
Anti-Calcium-Binding Protein Bdr-2 antibody
Anti-HPCA antibody
Anti-BDR2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance