| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-HIF1AN/FIH1 (1-100) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S3MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HIF1AN |
| Gene ID: |
55662 |
| Uniprot ID: |
HIF1N_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HIF1AN/FIH1 (Q9NWT6). |
| Immunogen Sequence: |
MAATAAEAVASGSGEPREEA GALGPAWDESQLRSYSFPTR PIPRLSQSDPRAEELIENEE PVVLTDTNLVYPALKWDLEY LQENIGNGDFSVYSASTHKF |
| Function | Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. |
| Protein Name | Hypoxia-Inducible Factor 1-Alpha InhibitorFactor Inhibiting Hif-1Fih-1Hypoxia-Inducible Factor Asparagine Hydroxylase |
| Database Links | Reactome: R-HSA-1234174 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMainly Cytoplasmic LocalizationBut Interaction With Notch1 Results In Nuclear Localization And Interaction With Abpa3 Results In Perinuclear Localization In Macrophages |
| Alternative Antibody Names | Anti-Hypoxia-Inducible Factor 1-Alpha Inhibitor antibodyAnti-Factor Inhibiting Hif-1 antibodyAnti-Fih-1 antibodyAnti-Hypoxia-Inducible Factor Asparagine Hydroxylase antibodyAnti-HIF1AN antibodyAnti-FIH1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
