Anti-HIC1 antibody (STJA0007757)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human HIC1
General Information
| Short Description | Rabbit polyclonal anti-Hypermethylated in cancer 1 protein for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HIC1 |
| Gene ID | 3090 |
| Uniprot ID | HIC1_HUMAN |
| Immunogen | Synthesized peptide derived from human HIC1 |
| Specificity | This antibody detects endogenous levels of HIC1 at Human, Mouse |
Additional Info
| Post Translational Modifications | Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1. Sumoylated on Lys-333 by a PIAS family member, which enhances interaction with MTA1, positively regulates transcriptional repression activity and is enhanced by HDAC4. |
| Function | Transcriptional repressor. Recognizes and binds to the consensus sequence '5-CGNGCGGGGCACACC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription of ACKR3, FGFBP1 and EFNA1. |
| Protein Name | Hypermethylated In Cancer 1 ProteinHic-1Zinc Finger And Btb Domain-Containing Protein 29 |
| Database Links | Reactome: R-HSA-3232118 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Hypermethylated In Cancer 1 Protein antibodyAnti-Hic-1 antibodyAnti-Zinc Finger And Btb Domain-Containing Protein 29 antibodyAnti-HIC1 antibodyAnti-ZBTB29 antibody |
Information sourced from Uniprot.org