• Immunofluorescence analysis of paraffin-embedded Human kidney tissue using HEXA Rabbit polyclonal antibody (STJ11104537) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of paraffin-embedded Rat kidney tissue using HEXA Rabbit polyclonal antibody (STJ11104537) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using HEXA Rabbit polyclonal antibody (STJ11104537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using HEXA Rabbit polyclonal antibody (STJ11104537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HEXA antibody (270-529) (STJ11104537)

SKU:
STJ11104537

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Beta-Hexosaminidase Subunit Alpha (270-529) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HEXA
Gene ID: 3073
Uniprot ID: HEXA_HUMAN
Immunogen Region: 270-529
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-529 of human HEXA (NP_000511.2).
Immunogen Sequence: IPGLLTPCYSGSEPSGTFGP VNPSLNNTYEFMSTFFLEVS SVFPDFYLHLGGDEVDFTCW KSNPEIQDFMRKKGFGEDFK QLESFYIQTLLDIVSSYGKG YVVWQEVFDNKVKIQPDTII QVWREDIPVNYMKELELVTK AGFRALLSAPWYLNRISYGP DWKDFYIVEPLAFEGTPEQK ALVIGGEACMWGEYVDNTNL VPRLWPRAGAVAERLWSNKL TSDLTFAYERLSHFRCELL
Post Translational Modifications N-linked glycan at Asn-115 consists of Man(3)-GlcNAc(2) (Probable). N-linked glycan at Asn-157 consists of either GlcNAc or GlcNAc(2)-Man(7-9). N-linked glycan at Asn-295 consists of either GlcNAc, GlcNAc-Fuc, or GlcNAc(2)-Man(4) (Probable).
Function Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A.
Protein Name Beta-Hexosaminidase Subunit Alpha
Beta-N-Acetylhexosaminidase Subunit Alpha
Hexosaminidase Subunit A
N-Acetyl-Beta-Glucosaminidase Subunit Alpha
Database Links Reactome: R-HSA-1660662
Reactome: R-HSA-2022857
Reactome: R-HSA-2024101
Reactome: R-HSA-2160916
Reactome: R-HSA-3656234
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Beta-Hexosaminidase Subunit Alpha antibody
Anti-Beta-N-Acetylhexosaminidase Subunit Alpha antibody
Anti-Hexosaminidase Subunit A antibody
Anti-N-Acetyl-Beta-Glucosaminidase Subunit Alpha antibody
Anti-HEXA antibody

Information sourced from Uniprot.org

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