• Western blot analysis of various lysates, using HDGF antibody (STJ11102694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates, using HDGF antibody (STJ11102694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using HDGF rabbit monoclonal antibody (STJ11102694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using HDGF rabbit monoclonal antibody (STJ11102694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HDGF antibody (100-200) [S4MR] (STJ11102694)

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STJ11102694

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HDGF (100-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDGF
Gene ID: 3068
Uniprot ID: HDGF_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HDGF (P51858).
Immunogen Sequence: YQSSQKKSCVEEPEPEPEAA EGDGDKKGNAEGSSDEEGKL VIDEPAKEKNEKGALKRRAG DLLEDSPKRPKEAENPEGEE KEAATLEVERPLPMEVEKNS T
Tissue Specificity Ubiquitous.
Post Translational Modifications Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. Phosphorylation at Ser-165 is likely to be required for secretion.
Function Isoform 1: Acts as a transcriptional repressor. Has mitogenic activity for fibroblasts. Heparin-binding protein. Isoform 2: Does not have mitogenic activity for fibroblasts. Does not bind heparin. Isoform 3: Has mitogenic activity for fibroblasts. Heparin-binding protein.
Protein Name Hepatoma-Derived Growth Factor
Hdgf
High Mobility Group Protein 1-Like 2
Hmg-1l2
Database Links Reactome: R-HSA-381038
Cellular Localisation Isoform 1: Nucleus
Cytoplasm
Secreted
Extracellular Exosome
Secreted By Exosomes And Is Located Inside The Exosome
May Also Be Secreted As Free Protein Via An As Yet Unknown Pathway
Isoform 2: Nucleus
Secreted By Exosomes And Is Located On The Outer Exosome Surface
Isoform 3: Nucleus
Alternative Antibody Names Anti-Hepatoma-Derived Growth Factor antibody
Anti-Hdgf antibody
Anti-High Mobility Group Protein 1-Like 2 antibody
Anti-Hmg-1l2 antibody
Anti-HDGF antibody
Anti-HMG1L2 antibody

Information sourced from Uniprot.org

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