• Western blot analysis of lysates from Human coronavirus (HCoV-HKU1) Nucleocapsid Protein (His Tag) , using HCoV-HKU1 Nucleoprotein Rabbit polyclonal antibody (STJ11103403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HCoV-HKU1 Nucleoprotein antibody (100-200) (STJ11103403)

SKU:
STJ11103403

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Host: Rabbit
Applications: WB/ELISA
Reactivity: HCoV-HKU1
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of coronavirus Nucleoprotein (YP_173242.1).
Immunogen Sequence: WYRHSRRSFKTADGQQKQLL PRWYFYYLGTGPYANASYGE SLEGVFWVANHQADTSTPSD VSSRDPTTQEAIPTRFPPGT ILPQGYYVEGSGRSASNSRP G
Background Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance