| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HCAR2 (165-182) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HCAR2 |
| Gene ID: |
338442 |
| Uniprot ID: |
HCAR2_HUMAN |
| Immunogen Region: |
165-182 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 165-182 of human GPR109A/HM74A/HCAR2 (NP_808219.1). |
| Immunogen Sequence: |
KKMPIQNGGANLCSSFSI |
| Tissue Specificity | Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils. |
| Function | Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. |
| Protein Name | Hydroxycarboxylic Acid Receptor 2G-Protein Coupled Receptor 109aG-Protein Coupled Receptor Hm74aNiacin Receptor 1Nicotinic Acid Receptor |
| Database Links | Reactome: R-HSA-3296197Reactome: R-HSA-373076Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Hydroxycarboxylic Acid Receptor 2 antibodyAnti-G-Protein Coupled Receptor 109a antibodyAnti-G-Protein Coupled Receptor Hm74a antibodyAnti-Niacin Receptor 1 antibodyAnti-Nicotinic Acid Receptor antibodyAnti-HCAR2 antibodyAnti-GPR109A antibodyAnti-HCA2 antibodyAnti-HM74A antibodyAnti-NIACR1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
