• Western blot analysis of extracts of various cell lines, using HAUS8 antibody (STJ110157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using HAUS8 antibody (STJ110157) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using HAUS8 antibody (STJ110157) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using HAUS8 antibody (STJ110157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HAUS8 antibody (166-410) (STJ110157)

SKU:
STJ110157

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAUS8 (166-410) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAUS8
Gene ID: 93323
Uniprot ID: HAUS8_HUMAN
Immunogen Region: 166-410
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 166-410 of human HAUS8 (NP_219485.1).
Immunogen Sequence: NLAEFERRAEKNLLIMCKEK EKLQKKAHELKRRLLLSQRK RELADVLDAQIEMLSPFEAV ATRFKEQYRTFATALDTTRH ELPVRSIHLEGDGQQLLDAL QHELVTTQRLLGELDVGDSE ENVQVLDLLSELKDVTAKKD LELRRSFAQVLELSAEASKE AALANQEVWEETQGMAPPSR WYFNQDSACRESGGAPKNTP LSEDDNPGASSAPAQATFIS PSEDFSSSSQAEVPPSLSR
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Protein Name Haus Augmin-Like Complex Subunit 8
Hec1/Ndc80-Interacting Centrosome-Associated Protein 1
Sarcoma Antigen Ny-Sar-48
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Spindle Pole
During Interphase
Primarily Cytoplasmic And Associates With Centrosomes And With The Mitotic Spindles
Preferentially At The Spindle Pole Vicinity
During Anaphase And Telophase
Additionally Associates With The Spindle Midzone And Midbody
Respectively
Localizes To Mitotic Spindle Microtubules
Alternative Antibody Names Anti-Haus Augmin-Like Complex Subunit 8 antibody
Anti-Hec1/Ndc80-Interacting Centrosome-Associated Protein 1 antibody
Anti-Sarcoma Antigen Ny-Sar-48 antibody
Anti-HAUS8 antibody
Anti-HICE1 antibody

Information sourced from Uniprot.org

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