• Immunofluorescence analysis of paraffin-embedded rat liver using HAO1/GOX Rabbit polyclonal antibody (STJ28553) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of HAO1/GOX in paraffin-embedded human liver cancer using HAO1/GOX Rabbit polyclonal antibody (STJ28553) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HAO1/GOX Rabbit polyclonal antibody (STJ28553) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HAO1 antibody (1-370) (STJ28553)

SKU:
STJ28553

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HAO1 (1-370) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HAO1
Gene ID: 54363
Uniprot ID: HAOX1_HUMAN
Immunogen Region: 1-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human HAO1/GOX (NP_060015.1).
Immunogen Sequence: MLPRLICINDYEQHAKSVLP KSIYDYYRSGANDEETLADN IAAFSRWKLYPRMLRNVAET DLSTSVLGQRVSMPICVGAT AMQRMAHVDGELATVRACQS LGTGMMLSSWATSSIEEVAE AGPEALRWLQLYIYKDREVT KKLVRQAEKMGYKAIFVTVD TPYLGNRLDDVRNRFKLPPQ LRMKNFETSTLSFSPEENFG DDSGLAAYVAKAIDPSISWE DIKWLRRLTSLPIVAKGIL
Tissue Specificity Highly expressed in liver.
Function Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine.this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones. Can also catalyze the oxidation of glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with much lower catalytic efficiency. Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2. Is not active on L-lactate and 2-hydroxybutanoate.
Protein Name 2-Hydroxyacid Oxidase 1
Haox1
Glycolate Oxidase
Go
Gox
Glyoxylate Oxidase
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome Matrix
Alternative Antibody Names Anti-2-Hydroxyacid Oxidase 1 antibody
Anti-Haox1 antibody
Anti-Glycolate Oxidase antibody
Anti-Go antibody
Anti-Gox antibody
Anti-Glyoxylate Oxidase antibody
Anti-HAO1 antibody
Anti-GOX1 antibody

Information sourced from Uniprot.org

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