| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-HADH/HADHSC (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 33kDa band observed in Human Heart, Muscle and Kidney lysates (calculated MW of 34.3kDa according to NP_005318.2). In transfected HEK293 transiently expressing HADH a band of a |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | HADH |
| Gene ID: | 3033 |
| Uniprot ID: | HCDH_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_005318.2 |
| Immunogen Sequence: | YERGDASKEDID |
| Post Translational Modifications | Succinylation at Lys-81, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5. |
| Function | Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion. Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes. |
| Protein Name | Hydroxyacyl-Coenzyme A Dehydrogenase - MitochondrialHcdhMedium And Short-Chain L-3-Hydroxyacyl-Coenzyme A DehydrogenaseShort-Chain 3-Hydroxyacyl-Coa Dehydrogenase |
| Database Links | Reactome: R-HSA-77310Reactome: R-HSA-77346Reactome: R-HSA-77348Reactome: R-HSA-77350Reactome: R-HSA-77352Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Hydroxyacyl-Coenzyme A Dehydrogenase - Mitochondrial antibodyAnti-Hcdh antibodyAnti-Medium And Short-Chain L-3-Hydroxyacyl-Coenzyme A Dehydrogenase antibodyAnti-Short-Chain 3-Hydroxyacyl-Coa Dehydrogenase antibodyAnti-HADH antibodyAnti-HAD antibodyAnti-HAD1 antibodyAnti-HADHSC antibodyAnti-SCHAD antibody |
Information sourced from Uniprot.org