• Immunofluorescence analysis of U2OS cells using GTF3C3 Rabbit polyclonal antibody (STJ11100359) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of H9C2 cells using GTF3C3 Rabbit polyclonal antibody (STJ11100359) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GTF3C3 in paraffin-embedded Mouse brain using GTF3C3 Rabbit polyclonal antibody (STJ11100359) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GTF3C3 in paraffin-embedded Rat brain using GTF3C3 Rabbit polyclonal antibody (STJ11100359) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GTF3C3 Rabbit polyclonal antibody (STJ11100359) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GTF3C3 antibody (1-160) (STJ11100359)

SKU:
STJ11100359

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTF3C3 (1-160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF3C3
Gene ID: 9330
Uniprot ID: TF3C3_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GTF3C3 (NP_036218.1).
Immunogen Sequence: MSGFSPELIDYLEGKISFEE FERRREERKTREKKSLQEKG KLSAEENPDDSEVPSSSGIN STKSQDKDVNEGETSDGVRK SVHKVFASMLGENEDDEEEE EEEEEEEEEEETPEQPTAGD VFVLEMVLNRETKKMMKEKR PRSKLPRALRGLMGEANIRF
Function Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
Protein Name General Transcription Factor 3c Polypeptide 3
Transcription Factor Iiic 102 Kda Subunit
Tfiiic 102 Kda Subunit
Tfiiic102
Transcription Factor Iiic Subunit Gamma
Tf3c-Gamma
Database Links Reactome: R-HSA-749476
Reactome: R-HSA-76061
Reactome: R-HSA-76066
Cellular Localisation Nucleus
Alternative Antibody Names Anti-General Transcription Factor 3c Polypeptide 3 antibody
Anti-Transcription Factor Iiic 102 Kda Subunit antibody
Anti-Tfiiic 102 Kda Subunit antibody
Anti-Tfiiic102 antibody
Anti-Transcription Factor Iiic Subunit Gamma antibody
Anti-Tf3c-Gamma antibody
Anti-GTF3C3 antibody

Information sourced from Uniprot.org

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