• Western blot analysis of extracts of SW480 cells, using GTF3A antibody (STJ110724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Western blot analysis of 293F, using GTF3A Rabbit polyclonal antibody (STJ110724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GTF3A antibody (1-100) (STJ110724)

SKU:
STJ110724

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTF3A (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF3A
Gene ID: 2971
Uniprot ID: TF3A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_002088.2).
Immunogen Sequence: MDPPAVVAESVSSLTIADAF IAAGESSAPTPPRPALPRRF ICSFPDCSANYSKAWKLDAH LCKHTGERPFVCDYEGCGKA FIRDYHLSRHILTHTGEKPF
Tissue Specificity Ubiquitous.
Function Involved in ribosomal large subunit biogenesis. Binds the approximately 50 base pairs internal control region (ICR) of 5S ribosomal RNA genes. It is required for their RNA polymerase III-dependent transcription and may also maintain the transcription of other genes. Also binds the transcribed 5S RNA's.
Protein Name Transcription Factor Iiia
Tfiiia
Database Links Reactome: R-HSA-749476
Reactome: R-HSA-76061
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Iiia antibody
Anti-Tfiiia antibody
Anti-GTF3A antibody

Information sourced from Uniprot.org

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