| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-GRP (1-148) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
GRP |
| Gene ID: |
2922 |
| Uniprot ID: |
GRP_HUMAN |
| Immunogen Region: |
1-148 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human Gastrin Releasing Peptide (GRP) (NP_002082.2). |
| Immunogen Sequence: |
MRGRELPLVLLALVLCLAPR GRAVPLPAGGGTVLTKMYPR GNHWAVGHLMGKKSTGESSS VSERGSLKQQLREYIRWEEA ARNLLGLIEAKENRNHQPPQ PKALGNQQPSWDSEDSSNFK DVGSKGKVGRLSAPGSQREG RNPQLNQQ |
| Function | Stimulates the release of gastrin and other gastrointestinal hormones. Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. In one study, shown to act in the amygdala as part of an inhibitory network which inhibits memory specifically related to learned fear. In another study, shown to act on vasoactive intestinal peptide (VIP)-expressing cells in the auditory cortex, most likely via extrasynaptic diffusion from local and long-range sources, to mediate disinhibition of glutamatergic cells via VIP cell-specific GRPR signaling which leads to enhanced auditory fear memories. Contributes to the regulation of food intake. Inhibits voltage-gated sodium channels but enhances voltage-gated potassium channels in hippocampal neurons. Induces sighing by acting directly on the pre-Botzinger complex, a cluster of several thousand neurons in the ventrolateral medulla responsible for inspiration during respiratory activity. Neuromedin-C: Induces an itch response through activation of receptors present on mast cells, triggering mast cell degranulation. |
| Protein Name | Gastrin-Releasing PeptideGrp Cleaved Into - Neuromedin-CGrp-10Grp18-27 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-381771Reactome: R-HSA-416476 |
| Cellular Localisation | SecretedCytoplasmic VesicleSecretory Vesicle LumenCell ProjectionNeuron ProjectionIn Neurons Of The Retrotrapezoid Nucleus/Parafacial Respiratory GroupExpressed On Neuron Projections Which Project Into The Pre-Botzinger Complex |
| Alternative Antibody Names | Anti-Gastrin-Releasing Peptide antibodyAnti-Grp Cleaved Into - Neuromedin-C antibodyAnti-Grp-10 antibodyAnti-Grp18-27 antibodyAnti-GRP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunohistochemistry analysis of EDF1 in paraffin-embedded mouse lung using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/99544/384668/STJ23470_1__14722.1713153436.jpg?c=1 "Immunohistochemistry analysis of EDF1 in paraffin-embedded mouse lung using [KO Validated] EDF1 Rabbit polyclonal antibody (STJ23470) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
