Anti-Granzyme B antibody (73-187) [ZR432] (STJ180648)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant human GZMB protein fragment (around aa 73-187)
General Information
| Short Description | Rabbit monoclonal Granzyme B (73-187) antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ZR432 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purified |
| Dilution Range | 1:100-200 |
| Formulation | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Storage Instruction | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
Target Information
| Gene Symbol | GZMB |
| Gene ID | 3002 |
| Uniprot ID | GRAB_HUMAN |
| Immunogen | Recombinant human GZMB protein fragment (around aa 73-187) |
| Immunogen Region | 73-187 |
| Specificity | Positive Control: Tonsil |
Additional Info
| Function | Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. |
| Protein Name | Granzyme BC11Ctla-1Cathepsin G-Like 1Ctsgl1Cytotoxic T-Lymphocyte Proteinase 2Lymphocyte ProteaseFragmentin-2Granzyme-2Human Lymphocyte ProteinHlpSectT-Cell Serine Protease 1-3e |
| Database Links | Reactome: R-HSA-2197563Reactome: R-HSA-5620971Reactome: R-HSA-75108Reactome: R-HSA-9725371 |
| Cellular Localisation | SecretedCytolytic GranuleDelivered Into The Target Cell By Perforin |
| Alternative Antibody Names | Anti-Granzyme B antibodyAnti-C11 antibodyAnti-Ctla-1 antibodyAnti-Cathepsin G-Like 1 antibodyAnti-Ctsgl1 antibodyAnti-Cytotoxic T-Lymphocyte Proteinase 2 antibodyAnti-Lymphocyte Protease antibodyAnti-Fragmentin-2 antibodyAnti-Granzyme-2 antibodyAnti-Human Lymphocyte Protein antibodyAnti-Hlp antibodyAnti-Sect antibodyAnti-T-Cell Serine Protease 1-3e antibodyAnti-GZMB antibodyAnti-CGL1 antibodyAnti-CSPB antibodyAnti-CTLA1 antibodyAnti-GRB antibody |
Information sourced from Uniprot.org