• Immunofluorescence analysis of U-2 OS cells using GPR22 antibody (STJ11100269) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using GPR22 antibody (STJ11100269) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GPR22 antibody (STJ11100269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GPR22 antibody (1-100) (STJ11100269)

SKU:
STJ11100269

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPR22 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPR22
Gene ID: 2845
Uniprot ID: GPR22_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GPR22 (NP_005286.2).
Immunogen Sequence: MCFSPILEINMQSESNITVR DDIDDINTNMYQPLSYPLSF QVSLTGFLMLEIVLGLGSNL TVLVLYCMKSNLINSVSNII TMNLHVLDVIICVGCIPLTI
Tissue Specificity High expression in adult and fetal heart tissue. Expressed in the brain, with enrichment in the accumbens, amygdala, cerebellum, cortex, and hippocampus regions.
Function Orphan G-protein coupled receptor. Seems to act through a G(i)/G(o) mediated pathway. May be involved in ciliogenesis.
Protein Name G-Protein Coupled Receptor 22
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-G-Protein Coupled Receptor 22 antibody
Anti-GPR22 antibody

Information sourced from Uniprot.org

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