Anti-GPR18 antibody (191-240 aa) (STJ93370)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GPR18 at the amino acid range 191-240
General Information
| Short Description | Rabbit polyclonal anti-N-arachidonyl glycine receptor (191-240 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPR18 |
| Gene ID | 2841 |
| Uniprot ID | GPR18_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GPR18 at the amino acid range 191-240 |
| Immunogen Region | 191-240 aa |
| Specificity | GPR18 Polyclonal Antibody detects endogenous levels of GPR18 protein. |
Additional Info
| Function | G protein-coupled receptor (GPCR) that plays a role in diverse physiological processes particularly within the immune and nervous systems. Becomes active when triggered by various endogenous ligands including endocannabinoid N-arachidonyl glycine (NAGly), delta-9-tetrahydrocannabinol or resolvin D2/RvD2 derived from the omega-3 fatty acid docosahexaenoic acid (DHA). Upon RvD2 binding, facilitates the resolution of inflammation, aiding in tissue repair and homeostasis. Mechanistically, RvD2 ligation initiates Galphas protein coupling, activation of cAMP-PKA signaling pathway and phosphorylation of STAT3, leading to RvD2-stimulated macrophage phagocytosis. Mediates NAGly-induced process of reorganization of actin filaments and induction of acrosomal exocytosis. Activation by N-arachidonoyl glycine (NAGly) can also induce apoptosis in macrophages. Plays a role in homeostasis of CD8+ subsets of intraepithelial lymphocytes (IELs) (CD8alphaalpha and CD8alphabeta IELs) in small intestine by supporting preferential migration of CD8alphaalpha T-cells to intraepithelial compartment over lamina propria compartment, and by mediating their reconstitution into small intestine after bone marrow transplant. Also participates in hypotensive responses, mediating reduction in intraocular and blood pressure. |
| Protein Name | N-Arachidonyl Glycine ReceptorNagly ReceptorG-Protein Coupled Receptor 18 |
| Database Links | Reactome: R-HSA-373076Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle Membrane |
| Alternative Antibody Names | Anti-N-Arachidonyl Glycine Receptor antibodyAnti-Nagly Receptor antibodyAnti-G-Protein Coupled Receptor 18 antibodyAnti-GPR18 antibodyAnti-GPCRW antibody |
Information sourced from Uniprot.org