![Western blot analysis of lysates from wild type (WT) and GPLD1 Rabbit polyclonal antibody knockdown (KD) 293T cells, using [KD Validated] GPLD1 Rabbit polyclonal antibody (STJ11104539) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177183/368686/STJ11104539_1__82427.1713134155.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GPLD1 Rabbit polyclonal antibody knockdown (KD) 293T cells, using [KD Validated] GPLD1 Rabbit polyclonal antibody (STJ11104539) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from Daudi cells, using [KD Validated] GPLD1 Rabbit polyclonal antibody (STJ11104539) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177183/368687/STJ11104539_2__55624.1713134156.jpg?c=1 "Western blot analysis of lysates from Daudi cells, using [KD Validated] GPLD1 Rabbit polyclonal antibody (STJ11104539) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and GPLD1 Rabbit polyclonal antibody knockdown (KD) 293T cells, using [KD Validated] GPLD1 Rabbit polyclonal antibody (STJ11104539) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177183/368686/STJ11104539_1__82427.1713134155.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GPLD1 Rabbit polyclonal antibody knockdown (KD) 293T cells, using [KD Validated] GPLD1 Rabbit polyclonal antibody (STJ11104539) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-GPLD1 antibody (24-160) (STJ11104539)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Phosphatidylinositol-Glycan-Specific Phospholipase D (24-160) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPLD1 |
| Gene ID | 2822 |
| Uniprot ID | PHLD_HUMAN |
| Immunogen Region | 24-160 |
| Immunogen Sequence | CGLSTHVEIGHRALEFLQLH NGRVNYRELLLEHQDAYQAG IVFPDCFYPSICKGGKFHDV SESTHWTPFLNASVHYIREN YPLPWEKDTEKLVAFLFGIT SHMAADVSWHSLGLEQGFLR TMGAIDFHGSYSEAHSA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2). |
Additional Info
| Function | This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. |
| Protein Name | Phosphatidylinositol-Glycan-Specific Phospholipase DPi-G PldGlycoprotein Phospholipase DGlycosyl-Phosphatidylinositol-Specific Phospholipase DGpi-PldGpi-Specific Phospholipase D |
| Database Links | Reactome: R-HSA-163125 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Phosphatidylinositol-Glycan-Specific Phospholipase D antibodyAnti-Pi-G Pld antibodyAnti-Glycoprotein Phospholipase D antibodyAnti-Glycosyl-Phosphatidylinositol-Specific Phospholipase D antibodyAnti-Gpi-Pld antibodyAnti-Gpi-Specific Phospholipase D antibodyAnti-GPLD1 antibodyAnti-PIGPLD1 antibody |
Information sourced from Uniprot.org