• Confocal imaging of paraffin-embedded Human colon cancer using GPA33 Rabbit monoclonal antibody (STJ11101910, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GPA33 Rabbit monoclonal antibody (STJ11101910) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GPA33 Rabbit monoclonal antibody (STJ11101910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-GPA33 antibody (220-319) [S0MR] (STJ11101910)

SKU:
STJ11101910

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GPA33 (220-319) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPA33
Gene ID: 10223
Uniprot ID: GPA33_HUMAN
Immunogen Region: 220-319
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 220-319 of human GPA33 (Q99795).
Immunogen Sequence: QFCNITVAVRSPSMNVALYV GIAVGVVAALIIIGIIIYCC CCRGKDDNTEDKEDARPNRE AYEEPPEQLRELSREREEED DYRQEEQRSTGRESPDHLDQ
Tissue Specificity Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Post Translational Modifications N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate. Palmitoylated.
Function May play a role in cell-cell recognition and signaling.
Protein Name Cell Surface A33 Antigen
Glycoprotein A33
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cell Surface A33 Antigen antibody
Anti-Glycoprotein A33 antibody
Anti-GPA33 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance