• Western blot analysis of extracts of various cell lines, using GNS antibody (STJ29625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GNS antibody (293-552) (STJ29625)

SKU:
STJ29625

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNS (293-552) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNS
Gene ID: 2799
Uniprot ID: GNS_HUMAN
Immunogen Region: 293-552
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 293-552 of human GNS (NP_002067.1).
Immunogen Sequence: WQTLLSVDDLVEKLVKRLEF TGELNNTYIFYTSDNGYHTG QFSLPIDKRQLYEFDIKVPL LVRGPGIKPNQTSKMLVANI DLGPTILDIAGYDLNKTQMD GMSLLPILRGASNLTWRSDV LVEYQGEGRNVTDPTCPSLS PGVSQCFPDCVCEDAYNNTY ACVRTMSALWNLQYCEFDDQ EVFVEVYNLTADPDQITNIA KTIDPELLGKMNYRLMMLQS CSGPTCRTPGVFDPGYRFD
Post Translational Modifications The form A (78 kDa) is processed by internal peptidase cleavage to a 32 kDa N-terminal species (form B) and a 48 kDa C-terminal species. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
Protein Name N-Acetylglucosamine-6-Sulfatase
Glucosamine-6-Sulfatase
G6s
Database Links Reactome: R-HSA-2022857
Reactome: R-HSA-2206305
Reactome: R-HSA-432720
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Alternative Antibody Names Anti-N-Acetylglucosamine-6-Sulfatase antibody
Anti-Glucosamine-6-Sulfatase antibody
Anti-G6s antibody
Anti-GNS antibody

Information sourced from Uniprot.org

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