• Immunofluorescence analysis of U-251MG cells using GNB3 Rabbit polyclonal antibody (STJ23822) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using GNB3 antibody (STJ23822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using GNB3 antibody (STJ23822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GNB3 antibody (1-340) (STJ23822)

SKU:
STJ23822

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNB3 (1-340) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNB3
Gene ID: 2784
Uniprot ID: GBB3_HUMAN
Immunogen Region: 1-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human GNB3 (NP_002066.1).
Immunogen Sequence: MGEMEQLRQEAEQLKKQIAD ARKACADVTLAELVSGLEVV GRVQMRTRRTLRGHLAKIYA MHWATDSKLLVSASQDGKLI VWDSYTTNKVHAIPLRSSWV MTCAYAPSGNFVACGGLDNM CSIYNLKSREGNVKVSRELS AHTGYLSCCRFLDDNNIVTS SGDTTCALWDIETGQQKTVF VGHTGDCMSLAVSPDFNLFI SGACDASAKLWDVREGTCRQ TFTGHESDINAICFFPNGE
Function Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Protein Name Guanine Nucleotide-Binding Protein G(I/G(S/G(T Subunit Beta-3
Transducin Beta Chain 3
Database Links Reactome: R-HSA-1296041
Reactome: R-HSA-163359
Reactome: R-HSA-202040
Reactome: R-HSA-381676
Reactome: R-HSA-381771
Reactome: R-HSA-392170
Reactome: R-HSA-392451
Reactome: R-HSA-392851
Reactome: R-HSA-400042
Reactome: R-HSA-4086398
Reactome: R-HSA-416476
Reactome: R-HSA-416482
Reactome: R-HSA-418217
Reactome: R-HSA-418555
Reactome: R-HSA-418592
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Reactome: R-HSA-420092
Reactome: R-HSA-428930
Reactome: R-HSA-432040
Reactome: R-HSA-456926
Reactome: R-HSA-500657
Reactome: R-HSA-6814122
Reactome: R-HSA-8964315
Reactome: R-HSA-8964616
Reactome: R-HSA-9009391
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Reactome: R-HSA-9717207
Reactome: R-HSA-997272
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(I/G(S/G(T Subunit Beta-3 antibody
Anti-Transducin Beta Chain 3 antibody
Anti-GNB3 antibody

Information sourced from Uniprot.org

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