![Immunofluorescence analysis of L929 cells using [KO Validated] GNAI3 Rabbit polyclonal antibody (STJ115271) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94305/375796/STJ115271_2__89678.1713142662.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] GNAI3 Rabbit polyclonal antibody (STJ115271) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and GNAI3 knockout (KO) HeLa cells, using [KO Validated] GNAI3 Rabbit polyclonal antibody (STJ115271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94305/375797/STJ115271_3__00724.1713142663.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GNAI3 knockout (KO) HeLa cells, using [KO Validated] GNAI3 Rabbit polyclonal antibody (STJ115271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KO Validated] GNAI3 Rabbit polyclonal antibody (STJ115271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94305/375798/STJ115271_4__20182.1713142663.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] GNAI3 Rabbit polyclonal antibody (STJ115271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-GNAI3 antibody (1-354) (STJ115271)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GNAI3 (1-354) antibody for use in WB, IF, ICC, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GNAI3 |
| Gene ID | 2773 |
| Uniprot ID | GNAI3_HUMAN |
| Immunogen Region | 1-354 |
| Immunogen Sequence | MGCTLSAEDKAAVERSKMID RNLREDGEKAAKEVKLLLLG AGESGKSTIVKQMKIIHEDG YSEDECKQYKVVVYSNTIQS IIAIIRAMGRLKIDFGEAAR ADDARQLFVLAGSAEEGVMT PELAGVIKRLWRDGGVQACF SRSREYQLNDSASYYLNDLD RISQSNYIPTQQDVLRTRVK TTGIVETHFTFKDLYFKMFD VGGQRSERKKWIHCFEGVTA IIFCVALSDYDLVLAEDEE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1). |
Additional Info
| Post Translational Modifications | (Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA. |
| Function | Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. The active GTP-bound form activates the calcium permeant TRPC5 ion channels. |
| Protein Name | Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3G(I Alpha-3 |
| Database Links | Reactome: R-HSA-170670Reactome: R-HSA-392170Reactome: R-HSA-418555Reactome: R-HSA-418594Reactome: R-HSA-418597Reactome: R-HSA-9009391Reactome: R-HSA-9634597Reactome: R-HSA-9660821 |
| Cellular Localisation | CytoplasmCell MembraneLipid-AnchorCytoskeletonMicrotubule Organizing CenterCentrosomeLocalizes In The Centrosomes Of Interphase And Mitotic CellsDetected At The Cleavage Furrow And/Or The Midbody |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3 antibodyAnti-G(I Alpha-3 antibodyAnti-GNAI3 antibody |
Information sourced from Uniprot.org