• Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GNAI1 antibody (STJ193040)

SKU:
STJ193040

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Guanine nucleotide-binding protein Gi subunit alpha-1 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNAI1
Gene ID: 2770
Uniprot ID: GNAI1_HUMAN
Specificity: GNAI1 Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein
Function Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis. Required for cortical dynein-dynactin complex recruitment during metaphase.
Protein Name Guanine Nucleotide-Binding Protein G(I Subunit Alpha-1
Adenylate Cyclase-Inhibiting G Alpha Protein
Database Links Reactome: R-HSA-170670
Reactome: R-HSA-392170
Reactome: R-HSA-400042
Reactome: R-HSA-418555
Reactome: R-HSA-418594
Reactome: R-HSA-418597
Reactome: R-HSA-422356
Reactome: R-HSA-9009391
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Cellular Localisation Nucleus
Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cell Cortex
Membrane
Lipid-Anchor
Localizes In The Centrosomes Of Interphase And Mitotic Cells
But Not In Centrosomes During Cytokinesis
Detected At The Cleavage Furrow Or The Midbody
Localized At The Plasma Membrane Throughout Mitosis
Colocalizes With Ric8a And Rgs14 At The Plasma Membrane
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein G(I Subunit Alpha-1 antibody
Anti-Adenylate Cyclase-Inhibiting G Alpha Protein antibody
Anti-GNAI1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance