| Host: | Rabbit |
| Applications: | IHC-P |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | IHC-P:1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GNA13 |
| Gene ID: | 10672 |
| Uniprot ID: | GNA13_HUMAN |
| Immunogen Region: | 1-377 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human GNA13 (NP_006563.2). |
| Immunogen Sequence: | MADFLPSRSVLSVCFPGCLL TSGEAEQQRKSKEIDKCLSR EKTYVKRLVKILLLGAGESG KSTFLKQMRIIHGQDFDQRA REEFRPTIYSNVIKGMRVLV DAREKLHIPWGDNSNQQHGD KMMSFDTRAPMAAQGMVETR VFLQYLPAIRALWADSGIQN AYDRRREFQLGESVKYFLDN LDKLGEPDYIPSQQDILLAR RPTKGIHEYDFEIKNVPFKM VDVGGQRSERKRWFECFDS |
| Tissue Specificity | Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level). |
| Post Translational Modifications | Palmitoylation is critical for proper membrane localization and signaling. Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation. |
| Function | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. |
| Protein Name | Guanine Nucleotide-Binding Protein Subunit Alpha-13 G Alpha-13 G-Protein Subunit Alpha-13 |
| Database Links | Reactome: R-HSA-193648 Reactome: R-HSA-416482 Reactome: R-HSA-428930 Reactome: R-HSA-456926 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Melanosome Cytoplasm Nucleus Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv Detected In The Cytoplasm Of Leydig Cells And In The Seminiferous Epithelium Including Differentiating Cells From The Spermatogonia To Mature Spermatozoa Stages In Round Spermatids Also Present In The Nuclei |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-13 antibody Anti-G Alpha-13 antibody Anti-G-Protein Subunit Alpha-13 antibody Anti-GNA13 antibody |
Information sourced from Uniprot.org