Anti-GNA11 antibody (STJ190861)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
STJ190861
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General Information

Short DescriptionRabbit polyclonal anti-Guanine nucleotide-binding protein subunit alpha-11 subunit alpha for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
ELISA 1:5000-20000
FormulationLiquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolGNA11
Gene ID2767
Uniprot IDGNA11_HUMAN
ImmunogenSynthesized peptide derived from part of the human protein
SpecificityGNA11 Polyclonal Antibody detects endogenous levels of protein.

Additional Info

Post Translational Modifications (Microbial infection) Deamidated at Gln-209 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
Function Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via phospholipase C-beta-dependent inositol lipid hydrolysis for signal propagation: activates phospholipase C-beta: following GPCR activation, GNA11 activates PLC-beta (PLCB1, PLCB2, PLCB3 or PLCB4), leading to production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs). Together with GNAQ, required for heart development. In the respiratory epithelium, transmits OXGR1-dependent signals that lead to downstream intracellular Ca(2+) release and mucocilliary clearance of airborne pathogens.
Protein Name Guanine Nucleotide-Binding Protein Subunit Alpha-11
G Alpha-11
G-Protein Subunit Alpha-11
Guanine Nucleotide-Binding Protein G(Y Subunit Alpha
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-202040
Reactome: R-HSA-399997
Reactome: R-HSA-416476
Reactome: R-HSA-418592
Reactome: R-HSA-428930
Reactome: R-HSA-434316
Reactome: R-HSA-456926
Reactome: R-HSA-6814122
Reactome: R-HSA-9856530
Reactome: R-HSA-9860927
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasm
In Testicular Cells
Expressed Exclusively In The Cytoplasm
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-11 antibody
Anti-G Alpha-11 antibody
Anti-G-Protein Subunit Alpha-11 antibody
Anti-Guanine Nucleotide-Binding Protein G(Y Subunit Alpha antibody
Anti-GNA11 antibody
Anti-GA11 antibody

Information sourced from Uniprot.org

Citations

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