Host: | Rabbit |
Applications: | WB/IHC-P/IF/ICC/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | GMNN |
Gene ID: | 51053 |
Uniprot ID: | GEMI_HUMAN |
Immunogen Region: | 1-209 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN (NP_056979.1). |
Immunogen Sequence: | MNPSMKQKQEEIKENIKNSS VPRRTLKMIQPSASGSLVGR ENELSAGLSKRKHRNDHLTS TTSSPGVIVPESSENKNLGG VTQESFDLMIKENPSSQYWK EVAEKRRKALYEALKENEKL HKEIEQKDNEIARLKKENKE LAEVAEHVQYMAELIERLNG EPLDNFESLDNQEFDSEEET VEDSLVEDSEIGTCAEGTVS SSTDAKPCI |
Post Translational Modifications | Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity. |
Function | Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing. Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control. |
Protein Name | Geminin |
Database Links | Reactome: R-HSA-68867Reactome: R-HSA-68962Reactome: R-HSA-69052 |
Cellular Localisation | CytoplasmNucleusMainly Cytoplasmic But Can Be Relocalized To The Nucleus |
Alternative Antibody Names | Anti-Geminin antibodyAnti-GMNN antibody |
Information sourced from Uniprot.org